Comparison of Muller‐Kauffmann tetrathionate broth with Rappaport‐Vassiliadis (RV) medium for the isolation of salmonellas from sewage sludge
- 1 February 1986
- journal article
- research article
- Published by Wiley in Journal of Applied Bacteriology
- Vol. 60 (2) , 161-167
- https://doi.org/10.1111/j.1365-2672.1986.tb03374.x
Abstract
The Rappaport-Vassiliadis (RV, modification of Rappaport''s) medium was superior to Muller-Kauffmann tetrathionate broth (MKT) for the enrichment and isolation of salmonellas from sewage sludge samples, either naturally contaminated or artificially inoculated with salmonella. Isolation rates for RV (42.degree. C) were higher even when MKT (43.degree. C) performance was improved by the use of brilliant green sulphamandelate agar. Kinetic results show that the suppression of the competing flora was poorer in MKT than in RV. The use of high inoculum to medium volume ratios (e.g. 1:5000) increased the isolation rates from RV and kinetic data explain why this was so.Keywords
This publication has 16 references indexed in Scilit:
- Growth kinetics of mixed culture in salmonella enrichment mediaJournal of Applied Bacteriology, 1985
- The effect of sodium sulphacetamide and sodium mandelate in brilliant green agar on the growth of salmonellasJournal of Applied Bacteriology, 1984
- Comparative study on Salmonella isolation from sewage—contaminated natural watersJournal of Applied Bacteriology, 1984
- Dynamics of salmonella isolation with modified Rappaport's medium (R10)Journal of Applied Bacteriology, 1983
- The Rappaport—Vassiliadis (RV) enrichment medium for the isolation of salmonellas: An overviewJournal of Applied Bacteriology, 1983
- Principles of Salmonella IsolationJournal of Applied Bacteriology, 1979
- A Modification of Brilliant Green Agar for Improved Isolation of SalmonellaJournal of Applied Bacteriology, 1978
- Isolation of Salmonellae from Sewage with a New Procedure of EnrichmentJournal of Applied Bacteriology, 1978
- Observations on Pre-enrichment for Isolating Salmonellas from Sewage Polluted Natural Water Using Muller-Kauffmann Tetrathionate Broth Prepared with Fresh and Desiccated Ox BileJournal of Applied Bacteriology, 1977
- Evaluation of the Growth of Salmonellae in Rappaport's Broth and in Muller‐Kauffmann's Tetrathionate BrothJournal of Applied Bacteriology, 1974