HPLC with Electrochemical and Fluorescence Detection of the OPA/2-Methyl-2-propanethiol Derivative of Fumonisin B1

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Abstract
The o-phthalaldehyde (OPA) derivative of fumonisin B1 was prepared in the presence of 2-methyl-2-propanethiol (tert-butyl thiol). A hydrodynamic voltammogram for the derivative indicated that the optimum voltage for maximum electrochemical response was ±0.7 V. The electrochemical response of the OPA/tert-butyl derivative was unstable. However, the fluorescence response was found to be stable for over an hour after an initial 30 minute reaction time. The minimum detectable limit (MDL) of the OPA/tert-butyl derivative using fluorescence detection was 30 ng/ml as compared to 250 ng/ml for electrochemical detection.