Histometric changes in connective fibers of the lung following paraquat administration in rats.
- 1 January 1993
- journal article
- Published by Japanese Society of Toxicology in The Journal of Toxicological Sciences
- Vol. 18 (3) , 167-170
- https://doi.org/10.2131/jts.18.3_167
Abstract
Pulmonary fibrosis induction by paraquat was studied histometrically using an image analyzer. Rats were given a subcutaneous injection of paraquat (7 or 25 mg/kg) and autopsied 3, 6 or 9 days later. The percentage of the area of connective fibers per unit area, and lung prolyl hydroxylase activity, as a biochemical parameter, were measured. Rats treated with 25 mg/kg of paraquat showed an increase in the percentage of the area of the connective fibers per unit area and also an increase in lung prolyl hydroxylase activity 6 days later. Thus, pulmonary fibrosis was confirmed both morphologically and biochemically. It is concluded that histometric examination using an image analyzer provides a very accurate method for the evaluation of pulmonary fibrosis.Keywords
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