CHARGE AND SIZE OF CONVERSION PRODUCTS OF SERUM BETA1C-GLOBULIN

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  • 1 January 1967
    • journal article
    • research article
    • Vol. 13  (6) , 649-+
Abstract
Using gel filtration on Sephadex G-200 combined with antigen-antibody crossed electro-phoresis of the fractions, serum [beta] 1C-globulin and the conversion products present in stored native serum and stored serum to which EDTA was added, were analyzed. The conversion products in the inter-[beta]- and gamma1-zone demonstrated in EDTA serum after 1 days storage and after hydrazine treatment, respectively, could not be characterized by gel filtration (probably because of this lability). Sephadex filtration did not induce any conversion of [beta] 1C-globulin in serum. The B1C- and [beta] 1A-globulin left the column in homogeneous peaks in the "7S" peak, the former slightly before the latter. The conversion product obtained in stored EDTA sera and on electrophoresis migrating in the [alpha]2-zone was found in the right extension of the macro-globulin peak. The precipitation lines produced by this component was diffuse and differed distinctly from the precipatation lines of the other components. The possibility of some kind of complex is discussed.