Cloning, nucleotide sequence and amplified expression of the gene encoding the extracellular metallo (Zn) DD-peptidase of Streptomyces albus G

1 September 1990

journal article
Published by Oxford University Press (OUP) in FEMS Microbiology Letters

Vol. 71 (1-2) , 215-220
https://doi.org/10.1016/0378-1097(90)90059-y

Abstract

The gene encoding the extracellular metallo (Zn) dd-peptidase of Streptomyces albus G has been cloned in Escherichia coli DH5αMCR via pBR322 or 325, and then transferred into Streptomyces lividans TK24 via pIJ486, with substantial amplification of the expressed dd-peptidase. The gene has the information for the synthesis of a 255 amino acid precursor, the amino terminal region of which has the characteristic features of a signal peptide. The primary structure as deduced from nucleotide sequencing confirms that previously determined by chemical methods except for the occurrence of an Asp instead of Asn at position 1 and an additional Ala immediately downstream of Pro67.

Keywords

This publication has 0 references indexed in Scilit: