The effect of 2,3‐diphosphoglycerate on the oxygen dissociation curve of human haemoglobin.

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Abstract
1. Oxygen dissociation curves for concentrated human haemoglobin solutions (1.6 mmol dm‐3 in haem) have been measured by mixing known quantities of oxy‐ and deoxyhaemoglobin solutions and measuring the resulting partial pressure of oxygen with an oxygen electrode. 2. Observations in the presence of 2,3‐diphosphoglycerate support previous conclusions derived from experiments at low haemoglobin concentrations, the validity of which has been questioned. 3. The two affinity state model of Monod, Wyman & Changeux (1965) does not fully describe the actions of 2,3‐diphosphoglycerate and a model in which this allosteric effector not only binds preferentially to the T state but also lowers the oxygen affinity of this state gives an improved fit to the data.