3' truncated tRNAArgis essential forin vitrospecific cleavage of partially synthesized mouse 18S rRNA
Open Access
- 1 January 1993
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 21 (20) , 4696-4702
- https://doi.org/10.1093/nar/21.20.4696
Abstract
An extensive analysis of oligonucleotide interactions was carried out by hybridising a synthetic pool of 256 10mers, A(C,T)gA, representing all oligopyrimidine octamer sequences to an array of four copies of all 256 different octapurine sequences. The resulting 256 duplexes were quantified by phosphorimaging and analysed to determine the dependence of duplex formation on base composition, sequence, and salt concentration. The results show that the base composition dependence of duplex formation can be reduced by high concentrations of tetramethylammonium chloride. This chaotropic solvent also increases duplex yield by up to fiftyfold.Keywords
This publication has 24 references indexed in Scilit:
- Role of arginine-tRNA in protein degradation by the ubiquitin pathwayNature, 1987
- [24] Analysis of modified nucleosides and nucleotide sequence of tRNAPublished by Elsevier ,1987
- Unusual priming mechanism of RNA-directed DNA synthesis in copia retrovirus-like particles of DrosophilaNature, 1986
- The RNA required in the first step of chlorophyll biosynthesis is a chloroplast glutamate tRNANature, 1986
- The E. coli dnaY gene encodes an arginine transfer RNACell, 1986
- Complete nucleotide sequence of mouse 18 S rRNA gene: comparison with other available homologsFEBS Letters, 1984
- Efficientin vitrosynthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoterNucleic Acids Research, 1984
- Bacteriophage T4-induced anticodon-loop nuclease detected in a host strain restrictive to RNA ligase mutants.Proceedings of the National Academy of Sciences, 1982
- Direct chemical method for sequencing RNA.Proceedings of the National Academy of Sciences, 1979
- The nucleotide sequen of human tRNAGly(anticodon GCC)Nucleic Acids Research, 1979