Specific Anti-Thyroxine Antisera Induced by Thyroxine Sensitized Liposomes
- 1 January 1981
- journal article
- research article
- Published by Taylor & Francis in Immunological Communications
- Vol. 10 (1) , 27-34
- https://doi.org/10.3109/08820138109050684
Abstract
Specific anti-thyroxine rabbit antisera were generated from complete Freund's adjuvant with liposomes consisting of sphingomyelin, cholesterol, dicetylphosphate and 5-N-thyroxine-2,4-dinitrophenyl-phosphatidylethanolamine (T4-Dnp-PE). Spin membrane immunoassay technique was used to measure the sensitivity and specificity of these antisera. Addition of 10 to 70 ng of Lthyroxine produced significant inhibition of immune lysis. Addition of L-3,3′ 5-triiodothyronine (T3) up to 800 ng showed no cross reaction.This publication has 5 references indexed in Scilit:
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