Survival of rat embryos after freezing

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Abstract
Effects of cryoprotectants and freeze-thawing procedures on the survival of frozen rat morulae were examined. In the samples frozen and thawed in the presence of DMSO [dimethylsulfoxide], ethylene glycol or glycerol, higher proportions of the embryos developed into blastocysts in culture when they were frozen slowly (50-71%) than when they were frozen rapidly (20-39%), but the thawing rates of the slowly frozen samples did not affect the viability of the embryos. When erythritol was used as a cryoprotectant, all of the embryos frozen-thawed slowly were killed but rapidly thawed embryos survived regardless of the freezing rate (36-52%). Morulae frozen slowly with ethylene glycol or glycerol and those frozen rapidly with DMSO or erythritol were transferred to recipients after thawing, and full-term young were obtained with all 4 cryoprotectants.

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