ACID-FASTNESS AS A HISTOCHEMICAL TEST
- 1 November 1953
- journal article
- research article
- Published by SAGE Publications in Journal of Histochemistry & Cytochemistry
- Vol. 1 (6) , 436-441
- https://doi.org/10.1177/1.6.436
Abstract
A study was made of the acid-fast reaction in cells and tissues, with the reaction in mycobacteria as the standard of comparison. For these bacilli, the reaction was obtained with all basic dyes. It was eliminated by pretreatment with 1 [image] HCl or with chromium-containing compounds but not by lipid extractions. The dye remaining in the cells after differentiation showed the same u.-v. absorption band which had been found to be unique and characteristic for the dye-mycolic acid-complex. Spermatozoa gave the same reactions as did tubercle and lepra bacilli. Keratohyaline granules and Russell bodies were acid-fast with all dyes but the acid-fastness was not altered by treatment with chromium compounds or acid nor was the 3500 A absorption peak present. Ceroid and epididymal pigment were acid-fast only with the fuchsins. The differences in the reactions of the acid-fast lipids in the cell and in the free state are descr.Keywords
This publication has 1 reference indexed in Scilit:
- Zur Chemie der Tuberkelbacillen.Hoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1899