Oxidising Enzymes

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Abstract
The authors have confirmed, within a certain range of H-ion concentration, the views of Czent-Gyorgyi that the oxidation product of catechol by the oxygenase of the potato tuber is capable of blueing guaiacum in the absence of peroxi-dase. Czent-Gyorgyi suggested that this oxidation product of catechol is orthoquinone, and his suggestion led the authors to the hypothesis that the oxidation of catechol may be a process of dehydrogenation, the oxygenase being a dehydrogenase. On this assumption, attempts were made to determine whether or not the oxygenase is capable of oxidizing catechol anaerobically in the presence of a H accepter by the use of methylene blue and others of the more readily reducible indicators, but the results were negative. It is shown qualitatively by tests with titanium sulphate that during the auto-oxidation of catechol, H2O2 is formed; and they conclude that it is probably also formed when the oxidation of catechol is catalyzed by oxygenase.

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