S-Adenosylethionine as an inhibitor of tRNA methylation

Publisher Website
Google Scholar
Abstract
S-Adenosylethionine was shown to inhibit the methylation of tRNA by S-adenosylmethionine. Lineweaver–Burk plots of kinetic data suggested that the inhibition was competitive. Another known inhibitor of tRNA methylation, adenine, also exhibited competitive inhibition kinetics. A derivative of S-adenosylethionine, ethylthioadenosine, also inhibited the reaction and displayed a competitive inhibition pattern. These data were obtained using Escherichia coli K12W6 as a source of methyl-deficient tRNA and methylase enzymes. Preliminary data indicate that S-adenosylethionine is even a better inhibitor of rat methylases.Methylase activity of ethionine-fed rats was elevated, which suggested that the inhibition reaction with S-adenosylethionine and the increased methylase activity may proceed by two different pathways.

This publication has 0 references indexed in Scilit: