An Improved Recombineering Approach by Adding RecA to λ Red Recombination
- 1 January 2006
- journal article
- Published by Springer Nature in Molecular Biotechnology
- Vol. 32 (1) , 043-054
- https://doi.org/10.1385/mb:32:1:043
Abstract
Recombineering is the use of homologous recombination in Escherichia colifor DNA engineering. Of several approaches, use of the λ phage Red operon is emerging as the most reliable and flexible. The...Keywords
This publication has 32 references indexed in Scilit:
- Recombineering with overlapping single-stranded DNA oligonucleotides: Testing a recombination intermediateProceedings of the National Academy of Sciences, 2003
- Engineering the mouse genome with bacterial artificial chromosomes to create multipurpose allelesNature Biotechnology, 2003
- Highly Efficient Modification of Bacterial Artificial Chromosomes (BACs) Using Novel Shuttle Vectors Containing the R6Kγ Origin of ReplicationGenome Research, 2002
- A comprehensive BAC resourceNucleic Acids Research, 2001
- Rapid engineering of bacterial artificial chromosomes using oligonucleotidesGenesis, 2000
- PCR-mediated gene replacement in Escherichia coliGene, 2000
- A new logic for DNA engineering using recombination in Escherichia coliNature Genetics, 1998
- Counterselectable markers: untapped tools for bacterial genetics and pathogenesis.1998
- Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector.Proceedings of the National Academy of Sciences, 1992
- An improved suicide vector for construction of chromosomal insertion mutations in bacteriaGene, 1992