EFFECT OF VASOPRESSIN ON SODIUM TRANSFER BY RAT COLONIN VITRO1

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Abstract
Everted segments of rat colon incubated in Krebs-Ringer bicarbonate containing glucose consistently show increased net transfer of sodium from mucosal to serosal fluid when Pitressin is present in the serosal fluid. When Pitressin is present only in the mucosal fluid, this effect is not observed. Blickenstaff (1) has reported an increase in water absorption from fistulas of jejunum in dogs after Pitressin injection, and Ussing (2) observed that the drug produced a transient increase in the short circuit current (active Na transport) across the isolated mucosa of the guinea pig cecum. The latter has also shown that Pitressin increases active transfer of sodium and flow of water across frog skin (3). Leaf (4) has reported a similar effect of Pitressin on active sodium transfer and water movement across isolated toad bladder. An in vitro method for study of sodium absorption by segments of rat intestine has been developed in this laboratory, and it seemed of interest to study the effect of vasopressin on this preparation.