Routine Direct Measurement of Arterial Pressure in Unanesthetized Rats

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Abstract
The method of permanent aortic intubation of Still et al. (Jour. Appl. Physiol. 8(5) 575-576. 1956) was adapted for routine use. Small size polyethylene tubing (PE 10) was inserted into the aorta, joined to larger (PE 50), brought to the back of the neck and out through the skin using 22 gauge hypodermic needle tubing. Needle tubing allowed replacement of the end piece of polyethylene tubing as necessary. Rats have been used as long as 6 months. Normal mean arterial pressure from 44 rats averaged 129[plus or minus]7.7 mm Hg. Simultaneous direct and indirect (photoelectric tensometer) measurements showed cuff placement markedly affected readings.