Quantitative RT-PCR Amplification of RNA in Single Mouse Oocytes and Preimplantation Embryos

Open Access

1 April 1998

journal article
Published by Taylor & Francis in BioTechniques

Vol. 24 (4) , 618-623
https://doi.org/10.2144/98244st02

Abstract

We describe a simple whole-cell method for quantitative reverse transcription (RT) PCR amplification of RNA that consistently allows the analysis of trace amounts of RNA, such as those carried by a...

Keywords

PROTEIN
PCR
MODELS
EMBRYOS
MOUSE
OOCYTE
HEAT
QUANTITATIVE
DNASE
PREIMPLANTATION

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