Male Germ Unit Isolation from Three Tricellular Pollen Species: Brassica oleracea, Zea mays, and Triticum aestivum
Open Access
- 1 March 1987
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 83 (3) , 464-466
- https://doi.org/10.1104/pp.83.3.464
Abstract
A technical procedure is described to follow the in vitro release of the `male germ unit' and the sperm cells from three tricellular pollen species (Brassica, Zea, and Triticum). The condition of the sperm cell was controlled using light microscopy. In addition, for the first time, the sperm cells viability has been checked by the fluorochromatic reaction test. These preliminary results indicate that this procedure appears to be a prerequisite for the successful preparation of purified viable sperm cells.This publication has 17 references indexed in Scilit:
- Preferential fertilization in Plumbago : Ultrastructural evidence for gamete-level recognition in an angiospermProceedings of the National Academy of Sciences, 1985
- An improved method to determine cell viability by simultaneous staining with fluorescein diacetate-propidium iodide.Journal of Histochemistry & Cytochemistry, 1985
- Applications of Fluorochromes to Pollen Biology. I. Mithramycin and 4′,6-Diamidino-2-Phenylindole (Dapi) as Vital Stains and for Quantitation of Nuclear DnaStain Technology, 1985
- Applications of Fluorochjromes to Pollen Biology. Ii. The Dna Probes Ethidium Bromide and Hoechst 33258 in Conjunction with the Callose-Specific Aniline Blue FluorochromeStain Technology, 1985
- Adenosine triphosphate in human semen: a quantitative estimate of fertilizing potentialFertility and Sterility, 1983
- Cellular nucleotide measurements and applications in microbial ecology.1980
- Isolation of vegetative and generative nuclei from pollen tubesExperimental Cell Research, 1972
- The Use of Fluorescein Diacetate and Phenosafranine for Determining Viability of Cultured Plant CellsStain Technology, 1972
- Evaluation of Pollen Viability by Enzymatically Induced Fluorescence; Intracellular Hydrolysis of Fluorescein DiacetateStain Technology, 1970
- Membrane properties of living mammalian cells as studied by enzymatic hydrolysis of fluorogenic esters.Proceedings of the National Academy of Sciences, 1966