Expression of Δ1‐pyrroline‐5‐carboxylate dehydrogenase and proline/arginine homeostasis in Solanum tuberosum
- 1 September 2000
- journal article
- Published by Wiley in Physiologia Plantarum
- Vol. 110 (1) , 22-27
- https://doi.org/10.1034/j.1399-3054.2000.110103.x
Abstract
Polyclonal antibodies raised in mouse against purified potato Δ1‐pyrroline‐5‐carboxylate dehydrogenase (P5C‐DH, EC 1.5.1.12), which catalyses the last step in the catabolism of both proline and arginine, were used to investigate the expression of this enzyme. Distribution of P5C‐DH in potato (Solanum tuberosum L. cv. Desiree) organs was studied at different stages during plant development. Variations in enzyme level were determined in axenically grown plantlets following the addition of exogenous proline, and in cell suspension cultures under hyperosmotic stress and after its relief. Free proline and arginine levels were also quantified, and compared to those of the enzyme. Results were consistent with a developmental, but not with an environmental, control of P5C‐DH expression. The possible involvement of specific isozymes in proline and arginine oxidation is discussed.Keywords
This publication has 21 references indexed in Scilit:
- Biochemical evidence for multiple acetoin-forming enzymes in cultured plant cells1Part 1 in the series ``Acetoin Synthesis in Higher Plants''.1Phytochemistry, 1999
- Reciprocal regulation of Δ 1-pyrroline-5-carboxylate synthetase and proline dehydrogenase genes controls proline levels during and after osmotic stress in plantsMolecular Genetics and Genomics, 1996
- Regulation of proline accumulation in Arabidopsis thaliana (L.) Heynh during development and in response to desiccationPlant, Cell & Environment, 1995
- Osmoregulation of a Pyrroline-5-Carboxylate Reductase Gene in Arabidopsis thalianaPlant Physiology, 1993
- Proline biosynthesis and osmoregulation in plantsThe Plant Journal, 1993
- Solubilization of a Proline Dehydrogenase from Maize (Zea mays L.) MitochondriaPlant Physiology, 1991
- Biochemical evidence for multiple forms of acetohydroxy acid synthase in Spirulina platensisArchiv für Mikrobiologie, 1991
- The Distribution of Free Amino Acids, Especially of Proline, in the Organs of Field Bean Plants, Vicia faba L., during Development in the FieldJournal of Plant Physiology, 1984
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- Rapid determination of free proline for water-stress studiesPlant and Soil, 1973