Regulation of nonspecific acid phosphatase in Salmonella: phoN and phoP genes

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Abstract
Mutations in S. typhimurium strains lacking nonspecific acid phosphatase mapped in 2 unlinked loci. One of these, phoP, was cotransducible by phage P22 with purB but the second, phoN, was cotransducible by phage P1 with purA. Mutants with temperature-sensitive nonspecific acid phosphatase activity (measured in whole cells) were also isolated. A phoN mutant with thermolabile whole-cell activity was isolated directly from wild-type LT-2. Several other mutants with temperature-sensitive enzyme activity were also isolated as revertants of phoN mutants. phoN might be a structural locus for nonspecific acid phosphatase. The observation that a mutation resulting in high levels of nonspecific acid phosphatase mapped in phoP suggests a possible regulatory role for this locus.