Direct Evidence for a Prostaglandin Receptor and Its Application to Prostaglandin Measurements

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Abstract
A prostaglandin receptor is present in rat adipocytes, as shown by competition studies with [ 3 H]-prostaglandin E 1 . The affinity of the E prostaglandins for this receptor preparation is greater than that of A and F prostaglandins, an observation consistent with their relative potencies in stimulating cyclic AMP formation in isolated mouse ovaries and various other organs. In the protocol described, binding constants of 3-8 nM were obtained with prostaglandins E 2 and E 1 . A sensitivity of 1 pmole makes this method readily applicable for measurement of tissue concentrations of the E prostaglandins.