Proline isomerization during refolding of ribonuclease A is accelerated by the presence of folding intermediates
- 31 March 1986
- journal article
- Published by Wiley in FEBS Letters
- Vol. 198 (2) , 217-220
- https://doi.org/10.1016/0014-5793(86)80408-2
Abstract
The trans → cis isomerization of Pro 93 was measured during refolding of bovine ribonuclease A. This isomerization is slow (τ = 500s) under marginally stable folding conditions of 2.0 M GdmCl, pH 6, at 10°C. However, it is strongly accelerated (τ = 100 s) in samples which, prior to isomerization, had been converted to a folding intermediate by a 15s refolding pulse under strongly native conditions (0.8 M ammonium sulfate, 0°C). The results demonstrate that extensive folding is possible before Pro 93 isomerizes to its native cis state and that the presence of structural folding intermediates leads to a marked increase in the rate of subsequent proline isomerization.Keywords
This publication has 20 references indexed in Scilit:
- Involvement of prolines-114 and -117 in the slow refolding phase of ribonuclease A as determined by isomer-specific proteolysisBiochemistry, 1984
- Isomerization of proline-93 during the unfolding and refolding of ribonuclease ABiochemistry, 1983
- Mechanism for the unfolding and refolding of ribonuclease A. Simulations using a simple model with no structural intermediatesBiochemistry, 1983
- Fast- and slow-refolding forms of unfolded ribonuclease A differ in tyrosine fluorescenceBiochemistry, 1982
- Identification and characterization of the direct folding process of hen egg-white lysozymeBiochemistry, 1982
- Nature of the fast and slow refolding reactions of iron(III) cytochrome cBiochemistry, 1981
- Acid catalysis of the formation of the slow-folding species of RNase A: Evidence that the reaction is proline isomerizationProceedings of the National Academy of Sciences, 1978
- Test of the extended two-state model for the kinetic intermediates observed in the folding transition of ribonuclease AJournal of Molecular Biology, 1978
- Consideration of the possibility that the slow step in protein denaturation reactions is due to cis-trans isomerism of proline residuesBiochemistry, 1975
- Both the Fast and Slow Refolding Reactions of Ribonuclease A Yield Native EnzymeProceedings of the National Academy of Sciences, 1973