Phages were isolated from Staphylococcus epidermidis after exposure of cultures to mitomycin C. Using certain of these phages, genes controlling resistance to novobiocin, streptomycin, and erythromycin and genes involved in the synthesis of adenine and riboflavin were transferred from donor to recipient strains of S. epidermidis. Interspecific transduction of the Novr marker was accomplished between S. epidermidis and S. aureus. Attempts to transfer penicillin resistance were not successful.