Abstract
Model systems, consisting of solutions of 2% gelatin, 2% gelatin and 6% glucose, and 9% skimmilk were inoculated with cultures of Pseudomonas, Escherichia coli, Serratia marcescens, Alcaligenes faecalis, Arthrobacter globiformis and Microbacterium lacticum. Viable counts of rehydrated freeze-dehydrated samples usually were highest in skimmilk. Reductions in viable count after rehydration with peptone or tryptone solutions were somewhat smaller than with distilled water. In general, the number of survivors was highest when rehydration was at 25 C with a volume of medium equal to that removed during freeze-dehydration. Rate of rehydration had a marked effect on the number of surviving bacteria. Highest numbers were found when rehydrated at a rate of 10−1 mg/sec/mg dry material.

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