INTRACELLULAR DESTRUCTION OF LEISHMANIA-TROPICA BY MACROPHAGES ACTIVATED WITH MACROPHAGE ACTIVATING FACTOR INTERFERON

  • 1 January 1984
    • journal article
    • research article
    • Vol. 55  (1) , 157-165
Abstract
Supernatants (SN) from cloned T cell lines [from mice], lymphokine secreting tumor cell lines [EL-4 thymoma cell, L929 fibroblast, WEHI-3-myelomonocyte leukemic cell] and mixed lymphocyte culture were analyzed for their ability to induce murine peritoneal exudate cell (PEC) macrophages (M.PHI.) parasitized with L. tropica to destroy the parasite. The ability of the SN to induce parasite destruction in parasitized PEC M.PHI. correlated with their content of macrophage activating factor/interferon. Neither granulocyte-macrophage colony stimulating factor, M.PHI. colony stimulating factor nor interleukin 2 was required for the induction of parasiticidal activity by infected PEC M.PHI.. Similar results were also obtained using either a different Leishmania spp., L. enriettii, or a 2nd source of cells: bone marrow derived macrophages.