Proteolytic denaturation and methods of improving the stability of glucose isomerase preparations
- 1 August 1979
- journal article
- research article
- Published by Wiley in Biotechnology & Bioengineering
- Vol. 21 (8) , 1345-1359
- https://doi.org/10.1002/bit.260210804
Abstract
Proteolytic denaturation of free and immobilized preparations of glucose isomerase from a Bacillus sp. is supported. Methods to improve the stability with respect to proteolysis were tested and their advantages and shortcomings are discussed. The methods include hollow-fiber treatment, gel permeation, thermal treatment and addition of protease inhibitors. The half-life of the free and the cellulose acetate fiber-entrapped preparations of glucose isomerase can be significantly improved; e.g., the hollow-fiber treatment can improve the half-life by an order of magnitude.This publication has 6 references indexed in Scilit:
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