Reversed-Phase Liquid Chromatography of Some Alkaloids

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Abstract
Retention data of 14 selected alkaloids were measured using an octadecylsilane stationary phase and methanol with aqueous buffers as the mobile phase. The effect of mobile phase composition (methanol content, pH, and buffer concentration) on the alkaloid capacity ratios was established. From the results obtained with different buffer concentrations and with different buffer salts, it can be concluded that the separation process is affected by the adsorption on free silanol groups.