Post‐translational modification of the β‐subunit of the human fibronectin receptor
Open Access
- 25 April 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 231 (2) , 402-406
- https://doi.org/10.1016/0014-5793(88)80859-7
Abstract
Monoclonal antibody DH12, directed against the β‐subunit of the fibronectin receptor recognizes a doublet of proteins (100 and 110 kDa) in Western blots of solubilized whole fibroblasts. Pulse‐chase experiments with [35S]methionine in human skin fibroblasts suggested that the two proteins might be metabolically related as precursor (100 kDa) and product (110 kDa). Endo H digestion and [3H]fucose labeling suggested that maturation converted the high‐mannose oligosaccharides (100 kDa) to the endoglycosidase H resistant complex type (110 kDa). This was supported by N‐glycanase digestion and by chemical deglycosylation which showed a single polypeptide. Surface iodination of intact cells labeled only the presumed mature β‐subunit.Keywords
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