Solid-Phase Artificial Chaperone-Assisted Refolding using Insoluble ?-Cyclodextrin?Acrylamide Copolymer Beads
- 1 December 2004
- journal article
- research article
- Published by Springer Nature in Biotechnology Letters
- Vol. 26 (23) , 1787-1791
- https://doi.org/10.1007/s10529-004-4609-6
Abstract
Solid-phase refolding methods are advantageous since they facilitate both separation of solid additives from the refolded protein and recycling of the additives. β-Cyclodextrin–acrylamide copolymer hydrogel beads were used as a matrix for detergents in solid-phase artificial chaperone-assisted refolding and improved the yield of lysozyme (up to 65%) and carbonic anhydrase B (up to 80%), compared with conventional solid host matrices.Keywords
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