Identiflcation of clones anE.colitRNAphegene by suppression of pheylananyl-tRNA synthetase thermosensitive mutants
Open Access
- 11 February 1983
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 11 (3) , 727-736
- https://doi.org/10.1093/nar/11.3.727
Abstract
Two libraries of cloned E.coli DNA were screened for plasmids which complemented thermosensitive phenylalanyl-tRNA synthetase mutants. Four plasmids were isolated which complemented pheS and pheT thermosen-sitive mutations but which do not carry pheS or pheT, the structural genes for phenylalanyl-tRNA synthetase. All these plasmids increased the intracellular tRNARne concentration. Three plasmids were shown to carry the structural gene for tRNA“ne which we call pheU. By restriction enzyme analysis, DNA blotting and DNA:tRNA hybridization, pheU was localised to a 280 bp fragment within a 5.6 kb PstIrestriction fragment of E.coli DNA.Keywords
This publication has 12 references indexed in Scilit:
- Cloning and mapping of a gene for translational initiation factor IF2 in Escherichia coli.Proceedings of the National Academy of Sciences, 1982
- Expression of the Gene for Escherichia coli Initiation Factor IF‐3 in vivo and in vitroEuropean Journal of Biochemistry, 1982
- A sensitive immunoblotting method for measuring protein synthesis initiation factor levels in lysates of Escherichia coli.Journal of Biological Chemistry, 1981
- Genes for the two subunits of phenylalanyl-tRNA synthetase of Escherichia coli are transcribed from the same promoterJournal of Molecular Biology, 1980
- Characterization of an E. coli mutant with a thermolabile initiation factor IF3 activityMolecular Genetics and Genomics, 1977
- A colony bank containing synthetic CoI EI hybrid plasmids representative of the entire E. coli genomeCell, 1976
- l‐Phenylalanyl‐tRNA Synthetase of Escherichia coli K‐10European Journal of Biochemistry, 1974
- The molecular weight and subunit composition of phenylalanyl-tRNA synthetase from Escherichia coli K-12Biochimie, 1974
- A rapid, sensitive, and specific method for the determination of protein in dilute solutionAnalytical Biochemistry, 1973
- The sequence of phenylalanine tRNA from E. coliFEBS Letters, 1969