Asparagine-linked sugar chains of fetuin: occurrence of tetrasialyl triantennary sugar chains containing the Gal.beta.1 .fwdarw. 3GlcNAc sequence
- 23 September 1986
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 25 (19) , 5709-5715
- https://doi.org/10.1021/bi00367a054
Abstract
Asparaginine-linked sugar chains were quantitatively released form fetuin by hydrazinolysis. Structural analysis of the sugar chains by sequential exoglycosidase digestion in combination with methylation analysis and Smith degradation revealed that most of them have typical biantennary (8%) and triantennary (74%) structures containing different amounts of N-acetylneuraminic acid residues. In addition, an unusual tetrasialyl triantennary sugar chain (17%) containing the Gal.beta.1 .fwdarw. 3 GlcNAc sequence in the outer chain moiety was detected, and its structure was elucidated as NeuAc.alpha.2 .fwdarw. 3 Gal.beta.1 .fwdarw. 3 (NeuAc.alpha.2 .fwdarw. 6)-GlcNAc.beta.1 .fwdarw. 4 (NeuAc.alpha.2 .fwdarw. 6 Gal.beta.1 .fwdarw. 4 GlcNAc.beta.1 .fwdarw. 2)Man.alpha.1 .fwdarw. 3(NeuAc.alpha.2 .fwdarw. 3Gal.beta.1 .fwdarw. 4GlcNAc.beta.1 .fwdarw. 2Man.alpha.1 .fwdarw. 6)Man.beta.1 .fwdarw. 4GlcNAc.beta.1 .fwdarw. 4GlcNAc.This publication has 15 references indexed in Scilit:
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