Horizontal gene transfer of atrazine‐degrading genes (atz) from Agrobacterium tumefaciens St96‐4 pADP1::Tn5 to bacteria of maize‐cultivated soil
- 19 July 2005
- journal article
- research article
- Published by Wiley in Pest Management Science
- Vol. 61 (9) , 870-880
- https://doi.org/10.1002/ps.1098
Abstract
The plasmid pADP1::Tn5 derived from pADP1[Atr+] carrying a Tn5 transposon conferring kanamycin and streptomycin resistances was constructed and introduced in Agrobacterium tumefaciens St96-4. This genetically modified strain was inoculated (∼108 cfu g−1) in potted soils planted with maize and treated or not with atrazine (1.5 mg kg−1). Bulk and maize rhizosphere soils were sampled 39 days after planting to look for soil indigenous bacteria that had acquired pADP1::Tn5. Four transconjugants were isolated from four different soil samples. The estimated transfer frequency of pADP1::Tn5 was 10−4 per donor. Maize rhizosphere and atrazine treatment had no obvious effect on pADP1::Tn5 transfer frequency. The sequencing of the 16S rDNA sequences of the transconjugants revealed that they were almost identical and highly similar to that of Variovorax spp (97%). In addition, their characterization suggested that the atzA and atzB genes had been transferred from pADP1::Tn5 to the bacterial chromosome in two of the four transconjugants. These data suggest that the atz degrading genes are horizontally transferred in soil and possibly subjected to gene rearrangement. Copyright © 2005 Society of Chemical IndustryKeywords
This publication has 63 references indexed in Scilit:
- Arthrobacter aurescens TC1 Atrazine Catabolism Genes trzN , atzB , and atzC Are Linked on a 160-Kilobase Region and Are Functional in Escherichia coliApplied and Environmental Microbiology, 2004
- Isolation and characterisation ofNocardioidessp. SP12, an atrazine-degrading bacterial strain possessing the genetrzNfrom bulk- and maize rhizosphere soilFEMS Microbiology Letters, 2003
- Synergistic Degradation of Linuron by a Bacterial Consortium and Isolation of a Single Linuron-Degrading Variovorax StrainApplied and Environmental Microbiology, 2003
- Enhanced degradation of phenoxyacetic acid in soil by horizontal transfer of the tfdA gene encoding a 2,4-dichlorophenoxyacetic acid dioxygenaseFEMS Microbiology Ecology, 2001
- Microbial aspects of atrazine biodegradation in relation to history of soil treatmentPesticide Science, 1999
- Accelerated Degradation and Mineralization of Atrazine in Surface and Subsurface Soil MaterialsPesticide Science, 1997
- The Role of Bioattenuation in the Management of Aromatic Hydrocarbon Plumes in AquifersGround Water Monitoring & Remediation, 1993
- Influence of soil type on the transfer of plasmid RP4p fromPseudomonas fluorescensto introduced recipient and to indigenous bacteriaFEMS Microbiology Letters, 1992
- Determination of plasmid transfer frequency in soil: Consequences of bacterial mating on selective agar mediaCurrent Microbiology, 1990
- Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectorsGene, 1985