Quantitative Micellar Electrokinetic Capillary Chromatography: Linear Dynamic Range

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Abstract
The linear dynamic range of micellar electrokinetic chromatography is affected by the concentration of the solute in the injection buffer. the data mimics a model for free solution capillary electrophoresis where the mobility of the analyte is less than the mobility of the buffer ions. This effect causes substantial peak dispersion in a concentration dependent fashion. Good linearity can be obtained by operating at low solute concentrations, e.g., less than 100 μg/mL for solutes such as naproxen, a non-steroidal anti-inflammatory agent. the linear dynamic range can be further improved by employing high ionic strength run buffers. to conpensate for the increase in Joule heating, 25 μm capillaries are employed. These effects are demonstrated with a series of non-steroidal antiinflammatory agents. At the 100 μg/mL level, the migration time % relative standard deviations (% RSD) range from 0.13 − 0.54%. Peak area % RSD's are approximately 1% using an external standard. Determination of several drugs in the tablet dosage form yield values of 101 − 104% of the labeled amount.