Binding of inositol phosphates and induction of Ca2+ release from pituitary microsomal fractions
- 1 June 1987
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 244 (2) , 493-496
- https://doi.org/10.1042/bj2440493
Abstract
Bovine anterior-pituitary microsomal fractions exhibit high-affinity, saturable and reversible binding of inositol 1,4,5-[32P]trisphosphate; 50% of the labelled ligand is displaced by 3.5 nM-inositol 1,4,5-trisphosphate. 0.5 microM-inositol 1,4-bisphosphate and 10 microM-ATP. Inositol 1,4,5-trisphosphate induces the release of Ca2+ from the microsomal vesicles (half-maximal effect at 290 nM), and its action is potentiated by inositol tetrakisphosphate (half-maximal effect at 4 microM).This publication has 33 references indexed in Scilit:
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