Comparison of two rapid culture methods for detection of cytomegalovirus in clinical specimens
- 1 December 1987
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 25 (12) , 2445-2446
- https://doi.org/10.1128/jcm.25.12.2445-2446.1987
Abstract
The conventional virus isolation technique was compared with a 24-h shell vial centrifugation culture technique and with a 48-h tube culture method for the detection of cytomegalovirus (CMV) in MRC-5 cells. Of 200 clinical specimens tested, 41 were positive for CMV by at least one procedure. Indirect immunoperoxidase staining was positive for 32 (78.0%) of 41 specimens in the tube culture method and for 30 (73.2%) of 41 specimens in the shell vial centrifugation method. CMV was detected in 23 (56.1%) of 41 specimens by the development of cytopathic effect within 14 days.This publication has 12 references indexed in Scilit:
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