SEROTYPING AVIAN ADENOVIRUSES BY A MICRO-NEUTRALIZATION PROCEDURE

  • 1 January 1977
    • journal article
    • research article
    • Vol. 38  (3) , 317-321
Abstract
A microneutralization procedure, using children kidney cell monolayers as an indicator system, was developed and applied to the serotyping of isolates characterized as avian adenoviruses. The method was reproducible, since coefficients of variation were low for 12 replicate titrations of homologous reagents of 9 prototype avian adenoviruses. Prototype reagents were specific according to results of reciprocal end point-neutralization tests and comparison of antigenic relatedness, using previous results. Avian adenovirus isolates (42) were classified into 6 serotypes by 1-sided end point-neutralization tests against antisera made to 9 prototype avian adenoviruses. An additional 20 isolates were antigenically related to prototype viruses, but they could not be specifically typed with the typing criteria. Different serotypes were isolated from birds having similar clinical diagnostic signs and lesions of disease.