Bacteria‐binding plasma proteins in pellicles formed on hydroxyapatite in vitro and on teeth in vivo

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Abstract
Previous studies on dental pellicle formation and bacterial adherence have focussed on saliva and its components. The tooth surface is, however, also exposed to the plasma‐derived crevicular fluid. In the present study, (i) plasma proteins in in vitro and in vivo pellicles were examined using sodium dodecyl sulphate‐polyacrylamide gel electrophoresis (SDS‐PAGE), immunoblotting and image analysis and (ii) the adherence of periodontopathogenic bacteria to experimental plasma and saliva pellicles was examined using radio‐labelled bacteria and liquid scintillation counting. The plasma components fibrinogen, fibronectin, albumin and IgG were incorporated from plasma in the experimental pellicle and mediated the adherence of Porphyromonas gingivalis, Fusobacterium nucleatum and Actinomycesnaeslundii. These proteins were also readily detected in in vivo pellicles and were found to a higher extent in pellicles formed at the gingival part of the tooth surface than at the incisal part.