STUDY OF GUINEA-PIG AND HUMAN-ANTIBODIES TO TOLUENE DIISOCYANATE

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Abstract
Antibodies to toluene diisocyanate (TDI) have been reported in sensitized guinea pigs and industrial workers. Detection of antibodies to TDI is difficult because the ability of TDI to self-polymerize and cross-link proteins causes problems in preparing satisfactory hapten-protein conjugate antigens. The use of monoisocyanate analogs of TDI for antigen preparation eliminates the problem. Three tolyl monoisocyanate isomers were compared with TDI for haptenic ability to detect antibodies to TDI in sensitized guinea pigs and humans. Using the gel-diffusion technique, antibodies from guinea pigs reacted with ortho-, meta- and para-tolyl monoisocyanate haptens and with a TDI hapten. In the guinea pigs, reactions appeared to be strongest with the TDI hapten and weakest with meta-tolyl isocyanate hapten. Antibodies in TDI-sensitized workers, as determined by radioallergosorbent testing, reacted comparably with each of the haptens.