Tumour inhibitory effects of TCGF/IL-2/-containing preparations

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Abstract
Supernatants from ConA-stimulated rat spleen cell cultures and from cultures of PMA-stimulated murine lymphoma subline EL-4TF were found to contain TCGF and to inhibit growth of a transplantable, MC-induced sarcoma MC11 in syngeneic mice. Tumor-inhibitory effects of the supernants were dependent on local and repeated administration. Prior to use of the supernatatants obtained from PMA-stimulated EL-4TF cell cultures, the dialysable PMA had to be removed; contamination with PMA was found to abolish the tumor-inhibitory effect of the superantants and to produce enhancement of tumour growth. A significant tumor-inhibitory effect has also been obtained with partially purified TCGF prepared from culture supernatants of cloned EL-4TF cells by ammonium sulphate precipitation, ion-exchange (FPLC) chromatography, and AcA 44 Ultrogel filtration.

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