Studies on Pituitary Follitropin. Iv. A Conformation Specific Radioimmunoassay for the Ovine Hormone
- 1 January 1978
- journal article
- research article
- Published by Taylor & Francis in Endocrine Research Communications
- Vol. 5 (4) , 279-291
- https://doi.org/10.1080/07435807809061093
Abstract
An antiserum to partially purified ovine follitropin (50 x NIH-FSH-S10) shows species specificity. It is conformation dependent and requires the proper recombination of the α and β subunits for maximal reactivity. The isolated α subunit is essentially inactive and the hormone specific β subunit is weakly reactive. The homologous radioimmunoassay is valuable for estimating native ovine follitropin in the presence of free subunits. It also provides a sensitive method to study association-dissociation and structure-function relationships.Keywords
This publication has 13 references indexed in Scilit:
- On the Isolation of Human Pituitary Hormones*Journal of Clinical Endocrinology & Metabolism, 1978
- Role of arginine residues in ovine lutropin: Reversible modification by 1,2-cyclohexanedioneArchives of Biochemistry and Biophysics, 1976
- Solvent exposure of aromatic groups in human luteinizing hormone and its subunits. Measurement by solvent perturbation difference spectroscopyBiochemistry, 1975
- Conformational Studies of Ovine Lutropin (Luteinizing Hormone) and Its Native and Chemically Modified Subunits by Circular Dichroism and Ultraviolet Absorption SpectroscopyEuropean Journal of Biochemistry, 1975
- The kinetics of dissociation and reassociation of ovine pituitary interstitial cell stimulating hormoneArchives of Biochemistry and Biophysics, 1974
- Rates of dissociation and recombination of the subunits of bovine thyrotropinArchives of Biochemistry and Biophysics, 1974
- Peptide HormonesAnnual Review of Biochemistry, 1974
- Interaction of 1,8-ANS with human luteinizing hormones: A probe for subunit interactions of hCG and hLHArchives of Biochemistry and Biophysics, 1973
- Circular dichroism of ovine interstitial cell stimulating hormone and its subunitsBiochemistry, 1972
- An immunochemical study of sheep pituitary interstitial cell-stimulating hormoneArchives of Biochemistry and Biophysics, 1961