Ploidy variability in greenhouse cultured and in vitro propagated potato (Solanum tuberosum) monohaploids (2n=x=12) as determined by flow cytometry

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Abstract
The DNA distributions of 23 different monohaploid potato clones were investigated by flow-cytometric measurements. All monohaploid clones differed in DNA distribution but none of them contained only monoploid cells in the leaves. All were highly stable on the monohaploid level for 2–3 years. Investigation of the influence of different factors on the DNA distribution in leaf cells showed that the material derived from in vitro shoot tip propagation contained a lower proportion of polyploidized cells than greenhouse grown plants. With protoplast isolation the enzyme treatment of in vitro cultured plant material induced a striking shift of DNA distribution towards the lower C-value whereas the mechanical purification steps caused a selective loss of monoploid nuclei. Seasonal influence on the DNA patterns could be detected.