Physical Map of the DNA of a Mamestra brassicae Nuclear Polyhedrosis Virus Variant Isolated from Spodoptera exigua

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Abstract
The DNA of a multiply-enveloped nuclear polyhedrosis virs (MNPV) of the cabbage moth Mamestra brassicae (Mb), isolated from the beet armyworm Sp. exigua, was analyzed using restriction endonucleases SmaI, BamHI, BamHI, Bg/II and PstI. The size of the viral DNA was calculated to be 152 kilobase pairs. Mapping of restriction sites on the DNA was accomplished by hybridization of 32P-labeled DNA fragments to single and double digests of the MbMNPV genome. Mapping of the putative polyhedrin gene was used to orient the physical map. The relevance of this map to the structural organization of the DNA of MbMNPV isolated from different geographical areas is discussed.

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