ON THE MECHANISM OF ACTION OF ALDOSTERONE ON SODIUM TRANSPORT: THE ROLE OF PROTEIN SYNTHESIS

Abstract
The hypothesis is presented that aldosterone regulates active Na+ transport by increasing de novo synthesis of enzymes involved in supplying energy for transport work. The evidence presented in support of this hypothesis included observations on: the relationships between the uptake of H3-aldosterone or its rate of removal and the time- course of action; nuclear localization of H3-aldosterone; specific inhibition of the action of aldosterone by actinomycin D and puromycin; and, elimination of the latent period by pretreatment of substrate-depleted bladders with aldosterone.