Detection of mRNA sequences in nuclear 30S ribonucleoprotein subcomplexes.

Publisher Website
Google Scholar
Abstract
RNA from nuclear 30S ribonucleoprotein (RNP) complexes of mouse ascites cells was shown to contain sequences homologous to poly(A) + mRNA by its ability to hybridize with complementary DNA [cDNA] prepared from poly(A) + mRNA template. Analysis of the hybridization kinetics of poly(A) + mRNA with its own complementary DNA revealed several abundancy classes. The total complexity of poly(A) + mRNA from ascites cells was estimated to be approximately 30,000 sequences of average MW (6 .times. 105). When the hybridization reaction of 30S RNP-RNA with mRNA-specific cDNA was compared to the homologous reaction the majority, and most probably all, of the poly(A) + mRNA sequences were found to be present in the RNA. The kinetics of hybridization suggest that 10-15% of the RNA complex is homologous to poly(A) + mRNA. The 30S RNP subcomplexes therefore contain nuclear poly(A) + mRNA sequences as well as the bulk of heterogeneous RNA.