PURIFICATION OF A PHYSIOLOGICAL FORM OF THE INHIBITOR PROTEIN OF THE CAMP-DEPENDENT PROTEIN-KINASE
- 1 January 1982
- journal article
- research article
- Vol. 257 (11) , 6028-6032
Abstract
The [rabbit muscle] inhibitor protein of the cAMP-dependent protein kinase exists in 2 forms which can be distinguished by separation techniques that characterize proteins on the basis of size. To date, only one of these forms has been purified and the form obtained is not the species that is present in the initial extracts of tissues. This paper reports the purification to homogeneity of the form of the inhibitor that is present in initial cell extracts and which, in consequence, appears to be a physiological size species of this protein.This publication has 11 references indexed in Scilit:
- Protein inhibitor of cAMP-dependent protein kinase: Production and characterization of antibodies and intracellular localizationCell, 1980
- Possibility of shape conformers of the protein inhibitor of the cyclic adenosine monophosphate-dependent protein kinaseBiochemistry, 1979
- A single amino acid substitution in a histidine-transport protein drastically alters its mobility in sodium dodecyl sulfate-polyacrylamide gel electrophoresisBiochemistry, 1979
- The protein inhibitor of adenosine 3': 5'-monophosphate-dependent protein kinasesBiochimie, 1979
- The protein inhibitor of adenosine 3′,5′-monophosphate-dependent protein kinases the NH2-terminal portion of the peptide chain contains the inhibitory siteBiochimica et Biophysica Acta (BBA) - General Subjects, 1979
- Sequence of two phosphorylated sites in the catalytic subunit of bovine cardiac muscle adenosine 3‘:5‘-monophosphate-dependent protein kinase.Journal of Biological Chemistry, 1979
- Autophosphorylation of rabbit skeletal muscle cyclic AMP-dependent protein kinase I catalytic subunit.Journal of Biological Chemistry, 1978
- Endogenous protein kinase inhibitors. Purification, characterization, and distribution in different tissues.Journal of Biological Chemistry, 1977
- Size and charge isomer separation and estimation of molecular weights of proteins by disc gel electrophoresisArchives of Biochemistry and Biophysics, 1968
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951