Adaptive Stress Response in Segmental Progeria Resembles Long-Lived Dwarfism and Calorie Restriction in Mice

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Abstract
How congenital defects causing genome instability can result in the pleiotropic symptoms reminiscent of aging but in a segmental and accelerated fashion remains largely unknown. Most segmental progerias are associated with accelerated fibroblast senescence, suggesting that cellular senescence is a likely contributing mechanism. Contrary to expectations, neither accelerated senescence nor acute oxidative stress hypersensitivity was detected in primary fibroblast or erythroblast cultures from multiple progeroid mouse models for defects in the nucleotide excision DNA repair pathway, which share premature aging features including postnatal growth retardation, cerebellar ataxia, and death before weaning. Instead, we report a prominent phenotypic overlap with long-lived dwarfism and calorie restriction during postnatal development (2 wk of age), including reduced size, reduced body temperature, hypoglycemia, and perturbation of the growth hormone/insulin-like growth factor 1 neuroendocrine axis. These symptoms were also present at 2 wk of age in a novel progeroid nucleotide excision repair-deficient mouse model (XPDG602D/R722W/XPA−/−) that survived weaning with high penetrance. However, despite persistent cachectic dwarfism, blood glucose and serum insulin-like growth factor 1 levels returned to normal by 10 wk, with hypoglycemia reappearing near premature death at 5 mo of age. These data strongly suggest changes in energy metabolism as part of an adaptive response during the stressful period of postnatal growth. Interestingly, a similar perturbation of the postnatal growth axis was not detected in another progeroid mouse model, the double-strand DNA break repair deficient Ku80−/− mouse. Specific (but not all) types of genome instability may thus engage a conserved response to stress that evolved to cope with environmental pressures such as food shortage. Oxidative damage to cellular components, including fats, proteins, and DNA, is an inevitable consequence of cellular energy use and may underlie both normal and pathological aging. Calorie restriction delays the aging process and extends lifespan in a number of lower organisms including rodents. Inborn defects in the postnatal growth axis resulting in dwarfism can also extend lifespan. Both may function via overlapping pathways impacting on energy metabolism. Here, we report a novel DNA repair-deficient mouse model with symptoms of the related premature aging disorders Cockayne syndrome and trichothiodystrophy, namely reduced fat deposits, neurological dysfunction, failure to thrive, and reduced lifespan. Surprisingly, we also observed traits usually associated with extended longevity as found in calorie restriction and dwarfism, including reduced blood sugar and reduced insulin-like growth factor-1. These characteristics were present at 2 wk of age, that is, during the period of rapid postnatal development, but returned to normal by sexual maturation at 10 wk. Furthermore, they were absent altogether in another premature aging mouse model with a distinct DNA repair defect. Specific types of unrepaired DNA damage may thus elicit a preservative organismal response affecting energy metabolism that is similar to the one that evolved to cope with the stress of food shortage.