Glutamate Synthase Isoforms in Rice

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Abstract
Rabbit antiserum was raised against ferredoxin-dependent glutamate synthase (EC 1.4.7.1) purified from green leaves of O. sativa L. cv. Delta. Ferredoxin-dependent glutamate synthase, detected in green leaf, etiolated leaf and root tissues cross-reacted completely with the anti-ferredoxin glutamate synthase Ig G. Immunoglobulin G did not cross-react with NADH-dependent (EC 1.4.1.14) and NADPH-dependent (EC 1.4.1.13) glutamate synthases found in nonphotosynthetic etiolated leaf and root tissues. Ferredoxin-dependent glutamate synthase was separated and distinguished by its affinity to ferredoxin from NAD(P)H-dependent glutamate synthase on ferredoxin-Sepharose affinity chromatography. Based on the immunological studies, ferredoxin-dependent glutamate synthases in green leaf and etiolated leaf tissues are closely related proteins; ferredoxin-dependent glutamate synthase in root tissue is a distinct protein from the leaf enzymes.