Colorimetric Determination of Tetracycline Hydrochloride in Pharmaceutical Preparations
- 1 July 1987
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of AOAC INTERNATIONAL
- Vol. 70 (4) , 686-688
- https://doi.org/10.1093/jaoac/70.4.686
Abstract
A simple, sensitive, and rapid colorimetric method is presented for the estimation of tetracycline hydrochloride as the pure drug and in formulations. The proposed method is based on the reaction of tetracycline HC1 with cupric chloride in an alkaline medium to give a yellowish green solution whose absorbance is measured at 400 nm against a reagent blank. The color obeys Beer's law in the concentration range of 0-20 μg/mL. Molar absorptivity and Sandell's sensitivity of the yellowish-green copper complexes of tetracycline HC1 are 1.99 x 104 Lmol-1 cm-1 and 0.0241 ppm, respectively.This publication has 1 reference indexed in Scilit:
- The Assay for Tetracycline HC1 and Chlortetracycline HCl in Tetracycline HC1Journal of the American Pharmaceutical Association (Scientific ed.), 1956