The coding sequence for the human 18,000-dalton hydrophobie pulmonary surfactant protein is located on chromosome 2 and identifies a restriction fragment length polymorphism
- 1 January 1988
- journal article
- research article
- Published by Springer Nature in Somatic Cell and Molecular Genetics
- Vol. 14 (1) , 105-110
- https://doi.org/10.1007/bf01535054
Abstract
The 18-kd Hydrophobic pulmonary surfactant protein (PSP-B) is a developmentally regulated protein which is important for normal lung function. A complementary DNA probe for 221 NH2 terminal amino acids of PSP-B was used to determine the chromosomal location of this gene and identify a restriction fragment length polymorphism (RFLP). Southern blot hybridization to genomic DNA isolated from a panel of human-CHO somatic cell hybrids unambiguously maps this gene to chromosome 2. Human DNA cut with BamHI yields a RFLP with variable bands at 2.8 and 2.6 kb. Since there is a relative lack of polymorphic markers for chromosome 2, this sequence may be useful in linkage analysis.This publication has 37 references indexed in Scilit:
- Detection of specific sequences among DNA fragments separated by gel electrophoresisPublished by Elsevier ,2006
- Proteolipid in bovine lung surfactant: Its role in surfactant functionBiochemical and Biophysical Research Communications, 1986
- Human T-Cell Receptor α-Chain Genes: Location on Chromosome 14Science, 1985
- An electrophoretic and immunochemical characterization of human surfactant-associated proteinsBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1984
- Analysis of pulmonary surfactant apoproteins by isoelectric focusingBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1984
- Characterization of rabbit surfactant-associated proteinsBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1983
- Interaction of the lipid and protein components of pulmonary surfactant Role of phosphatidylglycerol and calciumBiochimica et Biophysica Acta (BBA) - Biomembranes, 1981
- Isolation of a major apolipoprotein of canine and murine pulmonary surfactant Biochemical and immunochemical characteristicsBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1981
- Chromosomal assignment of the gene for folylpolyglutamate synthetase to human chromosome 9Cytogenetic and Genome Research, 1980