Ultraviolet Microscopy ofCandida albicans

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Abstract
Balish, Edward(Argonne National Laboratory, Argonne, Ill.),and George Svihla. Ultraviolet microscopy ofCandida albicans. J. Bacteriol.92:1812–1820. 1966.—Yeast and mycelial strains ofCandida albicanswere grown in medium supplemented with sulfur amino acids in an effort to determine factors that control the morphology and pathogenicity of the organism. Ultraviolet microscopy revealed a greater concentration ofS-adenosylmethionine in the vacuoles of the mycelial phase than in those of yeast phases. Supplementation with amino acids greatly increased the concentration ofS-adenosylmethionine in the mycelial phase, and made these cells more sensitive to the lytic action of snail gut enzymes than two yeast phase strains. This indicates a difference in cell wall structure that may be related to the pathogenicity of the mycelial phase.