Detection of cell-mediated immunity to spermatozoa in mice and man by the leucocyte adherence-inhibition test

Abstract
A modification of the leukocyte adherence-inhibition test was developed to study cell-mediated immunity to spermatozoa. The test is highly reproducible and requires very low numbers of leukocytes. Deposition of spermatozoa in the genital tract of the female mouse by 1 normal mating is sufficient to sensitize the female to sperm antigens and the degree of sensitization is independent of the number of matings and the strain of the male, and is not enhanced by subsequent pregnancy.